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Product Info Summary
| SKU: | EK1533-RB |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rabbit |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rabbit GREM2 ELISA Kit PicoKine®
View all PRDC/GREM2 ELISA kits
SKU/Catalog Number
EK1533-RB
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rabbit GREM2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rabbit GREM2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rabbit GREM2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1533-RB)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R22-Q168
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1533-RB for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1533-RB is reactive to Grem2 in Rabbit samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1533-RB is guaranteed for ELISA in Rabbit by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PRDC/GREM2
GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1533-RB-CAP | Anti-Rabbit Grem2 Pre-coated 96-well strip microplate | 1 |
| EK1533-RB-ST | Rabbit Grem2 Standard | 2 vials, 10 ng/tube |
| EK1533-RB-DA | Rabbit Grem2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.015 | 0.088 | 0.156 | 0.243 | 0.502 | 0.898 | 1.605 | 2.259 |
Data Example Images
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Rabbit GREM2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rabbit GREM2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 493 | 1462 | 2375 | 507 | 1583 | 2456 |
| Standard deviation | 17.25 | 73.1 | 114 | 31.94 | 125.06 | 184.2 |
| CV (%) | 3.5 | 5 | 4.8 | 7.9 | 7.9 | 7.5 |
Protein Target Info & Infographic
Gene/Protein Information For Grem2 (Source: Uniprot.Org, NCBI)
Gene Name
Grem2
Full Name
Gremlin-2
Weight
19.334kDa
Superfamily
DAN family
Alternative Names
CKTSF1B2; CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3; DAND3Prdc; GREM2; gremlin 2; gremlin 2, cysteine knot superfamily, homolog; gremlin-2; PRDC; PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis); Protein related to DAN and cerberus Grem2|Gremlin2, Pr, Prdc|gremlin 2, DAN family BMP antagonist|gremlin-2|Gremlin1|cysteine knot superfamily 1, BMP antagonist 2|gremlin 2 homolog, cysteine knot superfamily|protein related to DAN and cerberus
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Grem2, check out the Grem2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Grem2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rabbit GREM2 ELISA Kit PicoKine® (EK1533-RB)
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No publications found for EK1533-RB
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
10 Customer Q&As for Rabbit GREM2 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Rabbit PRDC Picokine® ELISA Kit (Catalog # EK1533-RB)?
M. King
Verified customer
Asked: 2020-12-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of PRDC causing disruption of its protein structure. PRDC may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PRDC ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-18
Question
Q: can you suggest the dilution ratio of serum samples for detection of PRDC in Rabbit serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-08-31
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PRDC ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-08-31
Question
Q: which procedure should I follow in order to thaw whole blood sample for PRDC ELISA after freezing?
K. Yang
Verified customer
Asked: 2020-08-16
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PRDC for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-08-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
N. Liu
Verified customer
Asked: 2020-06-23
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-06-23
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
W. Varadkar
Verified customer
Asked: 2020-04-18
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-18
Question
Q: how do I analyze ELISA data? I measured PRDC level in serum.
Verified Customer
Verified customer
Asked: 2020-02-23
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-23
Question
Q: What is the optimal O.D. value for PRDC ELISA kit? I used your PRDC ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PRDC even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PRDC ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of PRDC that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2019-10-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-08-24
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PRDC using the EK1533-RB Rabbit PRDC Picokine® ELISA Kit?
R. Kulkarni
Verified customer
Asked: 2019-03-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-03-13
Question
Q: Can PRDC ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-11-16
